Quite A few things affect transfection effectiveness and/or cellular Demise including the form and amount of transfection reagent, cell confluency, DNA amount and incubation time Using the reagent:DNA complex. Every of these elements will need to be optimized for each cell line-plasmid mix transfected in order to minimize cell Dying https://x.com/herringwileych1/status/1833482217064173936